FastQCFastQC Report
Thu 2 Jun 2016
DRR015442_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015442_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14234026
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185930.13062361976857426No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147330.10350550153554589No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA248100.037.1037831
GTATCAA410350.035.3075561
ATCAACG476850.029.9351733
TCAACGC478450.029.8731124
CAACGCA497300.028.7316275
TATCAAC505800.028.176822
AACGCAG524100.027.4763016
GTACATG453750.024.9406761
CGCAGAG576700.024.4465128
ACGCAGA581600.024.3765227
TACATGG466050.023.4329932
ACATGGG472250.022.412323
GCAGAGT629500.021.4705459
AGAGTAC515400.019.2552210-11
GAGTACT305050.018.90942212-13
CAGAGTA607500.017.65025310-11
GTACTTT321850.017.64645214-15
CATGGGG270650.017.5694164
TACCTGG109950.016.8855022
AGTACTT318550.016.87990212-13
Produced by FastQC (version 0.11.5)