FastQCFastQC Report
Thu 2 Jun 2016
DRR015442_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015442_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14234026
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160890.11303197001326258No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA370200.035.6066321
GGTATCA223700.033.388931
ATCAACG464550.027.931343
TCAACGC467450.027.7990264
CAACGCA482900.026.8097215
TATCAAC492450.026.4877472
AACGCAG511300.025.4095826
GTACATG415650.024.9512021
TACATGG424000.023.6240042
ACATGGG437800.022.1712023
CGCAGAG583600.021.8610618
ACGCAGA588050.021.8609927
AGAGTAC524050.019.85041610-11
GCAGAGT642000.019.3407259
AGTACTT336200.017.64723812-13
CATGGGG249250.017.5612724
GAGTACT320100.016.67211212-13
CAGAGTA624650.015.7026859
TACCTGG108700.015.6447492
GTACTTT336150.015.5964714-15
Produced by FastQC (version 0.11.5)