FastQCFastQC Report
Thu 2 Jun 2016
DRR015441_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015441_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2396634
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35170.146747480007377No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26880.11215730061411129No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33100.052.5124441
GTATCAA61200.040.9663241
ATCAACG72850.033.6031153
TCAACGC73300.033.396824
CAACGCA77200.031.6507365
TATCAAC77400.031.5101662
AACGCAG80650.030.6321146
ACGCAGA93500.026.3249267
CGCAGAG93200.026.3087398
GTACATG77950.023.7577381
GCAGAGT101300.023.4416529
TACATGG77550.023.3522172
GTGGTAT15650.022.0966321
ACATGGG79950.021.968263
GAGTACT53550.021.11006512-13
CAGAGTA97450.020.04972610-11
TGGTATC16000.019.6225742
GTACTTT58650.018.96395514-15
AGAGTAC93900.018.94254310-11
AGTACTT57600.017.84878212-13
Produced by FastQC (version 0.11.5)