FastQCFastQC Report
Thu 2 Jun 2016
DRR015441_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015441_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2396634
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT184890.7714569683981785No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT91270.3808257748158459RNA PCR Primer, Index 31 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT63650.2655808104199473RNA PCR Primer, Index 31 (95% over 24bp)
CATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCT31470.13130916109844057RNA PCR Primer, Index 31 (96% over 27bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29000.12100304009706947No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29800.047.3091621
GTATCAA54000.041.653951
TCAACGC69850.032.1751174
ATCAACG70000.032.169953
CAACGCA72700.030.792485
AACGCAG76300.029.5781576
TATCAAC75950.029.5296842
ACGCAGA89200.024.9950687
CGCAGAG89500.024.6154948
GTACATG69900.023.3020481
TACATGG70200.022.2080522
GCAGAGT97950.021.5625329
ACATGGG72400.020.4628563
AGAGTAC89250.020.34915410-11
AGTACTT56600.018.8185312-13
GAGTACT54150.018.24095212-13
CAGAGTA93550.017.7110319
ATAATAC13450.017.623923
CATGGGG41750.016.8152714
TATACTG12950.016.5136135
Produced by FastQC (version 0.11.5)