FastQCFastQC Report
Thu 2 Jun 2016
DRR015440_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015440_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16460823
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241530.14673020905455336No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184430.1120417855170425No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA266100.039.7054671
GTATCAA466650.038.4670331
TCAACGC556100.031.8213064
ATCAACG558200.031.7097423
CAACGCA578850.030.633555
TATCAAC587600.030.130922
TACCTGG147800.029.2162692
AACGCAG612800.029.1937186
GTACCTG172350.025.9522611
GTACATG629000.025.7677351
TATAACG16600.025.766342
ACGCAGA687200.025.6093187
CGCAGAG702300.024.9260238
TACATGG651050.024.245042
ACCTGGG173600.023.9568353
ACATGGG684850.022.3707543
GCAGAGT760350.022.095169
CATGGGG391500.019.4445154
AGAGTAC660250.019.06140510-11
CATGGGA269950.018.3222664
Produced by FastQC (version 0.11.5)