FastQCFastQC Report
Thu 2 Jun 2016
DRR015440_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015440_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16460823
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221290.13443434754143216No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA445850.037.7280121
GGTATCA259050.034.8097341
TCAACGC559150.029.557934
ATCAACG563300.029.3862363
TACCTGG149350.029.09822
CAACGCA576650.028.6522125
TATCAAC594150.028.0063292
AACGCAG609500.027.2871326
GTACATG572350.025.6898291
GTACCTG172650.025.480491
ACCTGGG168950.024.669773
TACATGG591400.024.0344332
ACGCAGA697450.023.494287
CGCAGAG709200.022.9686188
ACATGGG628550.021.92463
TATAACG14400.020.9006542
GCAGAGT763400.020.735739
AGAGTAC653600.019.98867810-11
CATGGGG363550.018.3900954
AGTACTT368800.017.98083512-13
Produced by FastQC (version 0.11.5)