FastQCFastQC Report
Thu 2 Jun 2016
DRR015439_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015439_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17261613
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT279380.16185045974556375No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217820.1261875121403776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA232400.052.1012841
GTATCAA456300.043.3290331
ATCAACG546800.035.641773
TCAACGC551100.035.4132084
CAACGCA576400.033.8114475
TATCAAC586650.033.4146042
AACGCAG612150.032.0560266
CGCAGAG681000.028.2178138
ACGCAGA685150.028.2155917
GTACATG594850.025.403941
GCAGAGT754100.024.6859289
TACATGG599400.024.231812
ACATGGG613350.022.9388073
GAGTACT406950.020.11626412-13
AGAGTAC700900.019.98139810-11
CAGAGTA748900.019.57849310-11
GTGGTAT111200.019.3960481
CATGGGG322800.018.3393294
GTACTTT446050.018.12821414-15
AGTACTT440650.017.6383412-13
Produced by FastQC (version 0.11.5)