FastQCFastQC Report
Thu 2 Jun 2016
DRR015439_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015439_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17261613
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT257730.1493081787895488No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180300.10445142061752861No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA200350.050.2174341
GTATCAA420300.045.053031
ATCAACG540200.034.5973783
TCAACGC543700.034.35124
CAACGCA566250.032.961555
TATCAAC576300.032.6596032
AACGCAG601550.031.1483366
CGCAGAG692950.026.6529278
ACGCAGA697950.026.6054947
GTACATG573950.023.7940271
GCAGAGT773200.023.1743269
TACATGG580850.022.7328782
ACATGGG601500.021.3460163
AGAGTAC717900.020.64829410-11
AGTACTT452150.018.8333112-13
GAGTACT421200.018.53152512-13
CAGAGTA765150.017.60065310-11
GTGGTAT101850.017.337451
CATGGGG312350.016.919334
GTACTTT460300.016.6039314-15
Produced by FastQC (version 0.11.5)