FastQCFastQC Report
Thu 2 Jun 2016
DRR015438_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015438_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17087744
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258360.15119608533461176No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200200.11715999490629073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA219650.052.7009281
GTATCAA440800.042.7460481
ATCAACG523650.035.505013
TCAACGC525400.035.3521044
CAACGCA554300.033.533555
TATCAAC566550.032.9289552
AACGCAG586200.031.8919226
CGCAGAG655350.028.0029288
ACGCAGA660700.027.9101857
GTACATG599050.025.1494481
GCAGAGT726550.024.2440039
TACATGG597600.024.0683482
ACATGGG617150.022.406413
GTGGTAT104600.019.749521
GAGTACT387450.019.56676712-13
CAGAGTA713200.019.5024710-11
AGAGTAC675650.019.1993310-11
CATGGGG339000.018.6035524
GTACTTT414850.018.02189314-15
AGTACTT408450.017.15742712-13
Produced by FastQC (version 0.11.5)