FastQCFastQC Report
Thu 2 Jun 2016
DRR015438_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015438_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17087744
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236230.13824528270086442No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA202600.051.016431
GTATCAA408800.043.7802241
ATCAACG518700.034.0291983
TCAACGC522250.033.7724844
CAACGCA542300.032.4398355
TATCAAC558350.031.8660412
AACGCAG573800.030.7700026
ACGCAGA666550.026.2019567
CGCAGAG667100.025.9936168
GTACATG569550.024.8537121
TACATGG571850.023.7938142
GCAGAGT739000.022.7994639
ACATGGG594200.022.3538653
AGAGTAC683200.020.12813610-11
GAGTACT405650.018.37781312-13
AGTACTT429650.018.28871312-13
CAGAGTA724800.017.93993610-11
CATGGGG329350.017.6802434
GTACTTT429450.017.09741814-15
CATGGGA232400.016.3049664
Produced by FastQC (version 0.11.5)