FastQCFastQC Report
Thu 2 Jun 2016
DRR015437_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015437_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17575845
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246370.14017533723129671No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185850.10574171540543285No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA244450.046.221371
GTATCAA446700.040.3399541
ATCAACG526700.033.582063
TCAACGC528050.033.5134434
CAACGCA550900.032.0820855
TATCAAC565800.031.3417762
AACGCAG580050.030.6153076
ACGCAGA659750.026.461717
CGCAGAG663250.026.1815158
GTACATG577800.024.4849571
TACATGG581500.023.7813852
GCAGAGT727150.023.1043019
ACATGGG603800.022.2926253
AGAGTAC656100.019.30352810-11
GAGTACT383450.018.91674812-13
CAGAGTA705700.018.80737910-11
TACCTGG143350.018.7605672
CATGGGG362900.018.5830674
GTACCTG153250.018.4691141
GTACTTT405350.017.61959514-15
Produced by FastQC (version 0.11.5)