FastQCFastQC Report
Thu 2 Jun 2016
DRR015437_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015437_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17575845
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220590.12550747915676316No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA405550.041.6839371
GGTATCA214050.041.6518441
ATCAACG507300.032.7593573
TCAACGC509250.032.5998734
CAACGCA528200.031.384545
TATCAAC546550.030.5652732
AACGCAG553700.030.152846
ACGCAGA651050.025.2248617
CGCAGAG657450.024.8189878
GTACATG537050.023.9754731
TACATGG547700.022.6843592
GCAGAGT725500.021.782119
ACATGGG561600.021.4571883
AGAGTAC650600.019.90316410-11
TACCTGG147050.018.9443152
GTACCTG155900.018.789441
AGTACTT408450.017.5286112-13
GAGTACT386300.017.08444812-13
CATGGGG330850.016.951194
CAGAGTA704850.016.75047910-11
Produced by FastQC (version 0.11.5)