FastQCFastQC Report
Thu 2 Jun 2016
DRR015436_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015436_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18382694
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258200.1404581939948519No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205450.11176272639907948No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA267850.046.4808541
GTATCAA483700.041.1765631
TCAACGC580400.034.0731244
ATCAACG585150.033.8043063
CAACGCA611050.032.297015
TATCAAC634000.031.0561282
AACGCAG642400.031.021836
ACGCAGA719350.027.1973787
CGCAGAG730150.026.630038
GTACATG634900.024.3323541
GCAGAGT792150.023.586349
TACATGG629000.023.1498932
ACATGGG665700.021.2379363
GAGTACT403900.019.76675212-13
CAGAGTA772000.019.2991110-11
AGAGTAC714700.019.25506210-11
GTACTTT435050.018.12082114-15
CATGGGG431850.018.0709444
GTGGTAT131300.017.8827631
AGTACTT429850.017.2399612-13
Produced by FastQC (version 0.11.5)