FastQCFastQC Report
Thu 2 Jun 2016
DRR015436_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015436_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18382694
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229410.124796724571491No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA225850.045.463391
GTATCAA432600.043.145571
ATCAACG569850.032.464663
TCAACGC569400.032.435944
CAACGCA587950.031.3156135
AACGCAG618150.029.8887396
TATCAAC619500.029.8848552
ACGCAGA716400.025.4594357
CGCAGAG728000.024.877298
GTACATG599100.023.3698251
TACATGG595650.022.178072
GCAGAGT793600.022.1441339
ACATGGG631450.020.4873983
AGAGTAC719500.019.99318310-11
AGTACTT437100.018.35287712-13
GAGTACT409700.018.14161912-13
CATGGGG393500.017.6847424
CAGAGTA772650.017.23353210-11
GTACTTT443500.016.65393414-15
GTGGTAT119300.014.9566981
Produced by FastQC (version 0.11.5)