FastQCFastQC Report
Thu 2 Jun 2016
DRR015435_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015435_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15988933
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT325260.2034282087491392No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247720.15493216464163057No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA237250.053.979881
GTATCAA481050.042.4785961
TACCTGG151900.035.444082
ATCAACG571700.035.106473
TCAACGC576500.034.7904854
CAACGCA604600.033.19325
TATCAAC618900.032.6129342
GTACCTG173300.031.7235971
AACGCAG642450.031.4493336
TATAACG15700.030.7271982
ACCTGGG180250.028.8089313
CGCAGAG718150.027.702798
ACGCAGA723500.027.6558287
GTACATG659650.025.8583031
TACATGG650550.025.1848032
GCAGAGT793300.024.5605749
ACATGGG666500.023.6329653
GTGGTAT107500.021.3371351
ATAACGC24850.020.695163
CATGGGG329550.019.927914
Produced by FastQC (version 0.11.5)