FastQCFastQC Report
Thu 2 Jun 2016
DRR015435_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015435_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15988933
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT333010.2082753114294744No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250400.15660832402012065No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA228050.053.7752341
GTATCAA472300.043.2949261
ATCAACG584300.034.3820763
TCAACGC590750.034.0210084
TACCTGG155550.033.4673232
CAACGCA609500.033.0239875
TATAACG15850.032.2117042
TATCAAC629200.032.095192
GTACCTG170250.031.5139871
AACGCAG651450.031.1488936
ACCTGGG181050.027.5702363
CGCAGAG750250.026.6501648
ACGCAGA756900.026.5510857
GTACATG622600.024.7093661
TACATGG616450.023.9443972
GCAGAGT829350.023.7785859
ACATGGG629700.022.5863363
GTGGTAT103300.020.448611
AGAGTAC793800.020.11325310-11
GTATAAC41050.020.005181
Produced by FastQC (version 0.11.5)