FastQCFastQC Report
Thu 2 Jun 2016
DRR015434_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015434_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7394378
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144850.1958920682713272No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108920.14730109821272322No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG95600.042.0352552
GGTATCA136400.041.4729271
TATAACG9600.039.3475532
GTACCTG104800.038.7357061
GTATCAA238050.036.8401571
ACCTGGG114250.034.1776283
ATAACGC11700.031.5072143
ATCAACG281300.030.6099263
TCAACGC281550.030.5827454
CAACGCA295250.029.2391535
TATCAAC296150.029.167242
AACGCAG315350.027.7585496
GTACATG277200.025.7923471
TACATGG277200.025.0208822
CGCAGAG352000.024.6479518
ACGCAGA352550.024.571457
ACATGGG287500.023.1746983
GTATAAC25800.022.7550561
TCTGTCG18700.022.3830388
CCTGGGG76600.022.161084
Produced by FastQC (version 0.11.5)