FastQCFastQC Report
Thu 2 Jun 2016
DRR015434_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015434_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7394378
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147360.19928653904358146No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97520.13188397996423767No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83880.11343753321780412No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG86450.041.7022252
GTATCAA217650.039.796131
GTACCTG96600.038.245681
TATAACG9900.036.8300672
GGTATCA119900.035.3023261
ACCTGGG105600.033.664983
ATAACGC12300.032.978633
ATCAACG280950.030.4496943
TCAACGC284650.030.0267564
TATCAAC295300.029.1397782
CAACGCA293450.029.0625135
AACGCAG316000.027.305246
CGCAGAG361750.023.6171198
GTACATG235200.023.5620731
ACGCAGA364100.023.548537
TAACGCA17300.022.9240594
TACATGG237350.022.5629712
CCTGGGG71750.021.3469454
AGAGTAC344800.021.31860510-11
GCAGAGT391600.021.293129
Produced by FastQC (version 0.11.5)