FastQCFastQC Report
Thu 2 Jun 2016
DRR015433_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015433_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13673863
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228810.16733383974960112No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181820.13296900809961312No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA178850.052.41131
GTATCAA347500.042.3871841
TCAACGC412250.035.1337284
ATCAACG413650.035.0258183
CAACGCA430450.033.5940175
TATCAAC442150.032.9327962
AACGCAG454100.032.059816
ACGCAGA516850.027.797787
CGCAGAG516850.027.6124238
GTACATG430950.024.7290061
GCAGAGT574550.024.220379
TACATGG435950.023.5478172
ACATGGG457450.021.7646623
GAGTACT324600.020.4399512-13
AGAGTAC528500.019.96972710-11
CAGAGTA562050.019.78132410-11
GTACTTT351600.018.63025914-15
GTGGTAT82250.018.3121241
CATGGGG280350.018.2762074
AGTACTT340300.018.23368312-13
Produced by FastQC (version 0.11.5)