FastQCFastQC Report
Thu 2 Jun 2016
DRR015433_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015433_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13673863
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241330.1764899940857971No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173700.1270306715812496No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA173550.049.4256361
GTATCAA342000.042.8383981
ATCAACG421400.034.209783
TCAACGC423750.034.0130464
CAACGCA441000.032.7410135
TATCAAC450150.032.1362952
AACGCAG464600.031.2541876
ACGCAGA540200.026.7121377
CGCAGAG540800.026.5255368
GTACATG409800.023.8265321
GCAGAGT597400.023.4326559
TACATGG417150.022.8202932
ACATGGG429050.021.3903983
AGAGTAC549750.020.4843710-11
GAGTACT347700.019.17490812-13
AGTACTT369950.018.86431912-13
CAGAGTA581600.018.5566310-11
TATAACG13950.018.3019282
GTACTTT378450.017.3381314-15
GTGGTAT85400.017.2569371
Produced by FastQC (version 0.11.5)