FastQCFastQC Report
Thu 2 Jun 2016
DRR015432_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015432_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12490715
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222680.17827642372754482No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173520.13891918917371826No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA188350.049.2169151
GTATCAA361950.041.478471
ATCAACG422850.034.8699343
TCAACGC423800.034.7488174
CAACGCA443200.033.194845
TATCAAC453300.032.658092
AACGCAG465400.031.7703866
ACGCAGA521100.028.060177
CGCAGAG520600.027.946968
GTACATG441800.024.6493781
GCAGAGT577900.024.6233169
TACATGG439800.023.9442142
ACATGGG443400.022.7234553
GAGTACT319000.020.28564512-13
AGAGTAC528250.020.10420210-11
CAGAGTA572000.019.63222910-11
GTGGTAT90000.019.0628491
GTACTTT337650.018.72019414-15
AGTACTT332000.018.23072812-13
CATGGGG223150.017.681324
Produced by FastQC (version 0.11.5)