FastQCFastQC Report
Thu 2 Jun 2016
DRR015432_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015432_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12490715
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222330.17799621558893947No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166310.1331469015184479No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA184250.047.9600141
GTATCAA354950.041.8736761
ATCAACG436900.033.42963
TCAACGC440000.033.2203944
CAACGCA453750.032.1731265
TATCAAC465400.031.558762
AACGCAG476500.030.7612066
ACGCAGA548850.026.474187
CGCAGAG549750.026.2524098
GTACATG425050.023.8807091
GCAGAGT607200.023.3183449
TACATGG422800.023.0619772
ACATGGG426850.021.9460963
AGAGTAC559500.020.49069210-11
GAGTACT350250.019.18593812-13
TATAACG12700.019.023612
AGTACTT364350.018.66830312-13
CAGAGTA605700.018.1763610-11
GTACTTT365600.018.01067714-15
CATGGGG211150.017.5331064
Produced by FastQC (version 0.11.5)