FastQCFastQC Report
Thu 2 Jun 2016
DRR015431_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015431_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3526634
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54300.1539711804513879No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43550.12348885651303765No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56300.043.9698831
GTATCAA102150.040.7612531
ATCAACG120800.033.9413263
TCAACGC121800.033.587944
CAACGCA127300.032.0982935
TATCAAC128650.032.0117762
AACGCAG131800.031.0674556
ACGCAGA143350.028.310387
CGCAGAG143250.028.1705448
GCAGAGT157750.024.8584129
GTACATG104750.023.9800361
TACATGG105500.022.6021542
GAGTACT85600.021.76207212-13
ACATGGG108550.021.2544123
AGAGTAC135850.020.71154210-11
CAGAGTA156600.019.78279710-11
GTACTTT96000.019.26167314-15
AGTACTT90750.018.47187212-13
CATGGGT23050.017.570744
CATGGGG64200.016.5155054
Produced by FastQC (version 0.11.5)