FastQCFastQC Report
Thu 2 Jun 2016
DRR015431_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015431_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3526634
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT310940.8816905865479662No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT164370.4660818219299196RNA PCR Primer, Index 31 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT114200.3238215250008932RNA PCR Primer, Index 31 (95% over 24bp)
CATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCT62970.17855552915329462RNA PCR Primer, Index 31 (96% over 27bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50920.1443869706921671No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40510.11486873885977393No Hit
CACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTG35610.10097447027392124RNA PCR Primer, Index 38 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55500.043.647081
GTATCAA97500.037.2444951
TCAACGC119300.030.0761644
ATCAACG119400.029.9686433
CAACGCA121450.029.4863155
TATCAAC126650.028.5050452
AACGCAG127150.028.2718376
ACGCAGA145950.024.443047
CGCAGAG146150.024.1930228
GTACATG103900.023.5343861
TACATGG104850.022.5631982
ACATGGG104000.021.8271853
GCAGAGT160200.021.5306029
AGAGTAC140450.020.11945510-11
GAGTACT88150.019.66735512-13
AGTACTT94800.017.9997412-13
CAGAGTA158100.017.71505710-11
GTACTTT97400.017.37774314-15
CTGTCGC9350.016.5469049
CATGGGG59650.016.4329744
Produced by FastQC (version 0.11.5)