FastQCFastQC Report
Thu 2 Jun 2016
DRR015430_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015430_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10965406
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181050.16511016555155367No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138960.12672581389143275No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA141500.051.2619971
GTATCAA281550.042.571981
ATCAACG335850.035.2553943
TCAACGC337400.035.1878434
CAACGCA350600.033.7439085
TATCAAC366400.032.35312
AACGCAG374650.031.765876
ACGCAGA420300.028.0342627
CGCAGAG420850.027.8994468
TACCTGG92000.025.5226352
GCAGAGT459800.024.9021749
TATAACG11350.024.4566862
GTACATG340600.023.9818941
GTACCTG100150.023.9454671
TACATGG350450.022.3599452
ACATGGG355050.020.968023
AGAGTAC430800.020.46977610-11
CAGAGTA456500.019.6611710-11
GAGTACT279150.019.36768212-13
ATAACGC15500.019.0829413
Produced by FastQC (version 0.11.5)