FastQCFastQC Report
Thu 2 Jun 2016
DRR015430_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015430_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10965406
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181170.1652196006239988No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130820.11930246814390638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA137950.049.485781
GTATCAA272450.042.59221
ATCAACG333000.034.190793
TCAACGC335650.033.8856744
CAACGCA346400.032.8948825
TATCAAC364250.031.4577582
AACGCAG368600.031.1976456
ACGCAGA424050.026.9576767
CGCAGAG425950.026.798858
TACCTGG85850.026.0781062
GCAGAGT470550.023.8037389
GTACCTG97800.023.5159321
GTACATG318350.023.2926581
TATAACG12050.022.7038252
TACATGG324300.022.018092
ACATGGG329000.020.7749773
AGAGTAC437350.020.57391210-11
ACCTGGG115550.019.0200613
AGTACTT297350.018.91863612-13
GAGTACT290200.018.65547612-13
Produced by FastQC (version 0.11.5)