FastQCFastQC Report
Thu 2 Jun 2016
DRR015429_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015429_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14748823
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT296340.2009245076708833No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229440.15556495592902567No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA256650.047.1648031
GTATCAA478550.040.481261
TCAACGC576700.033.228674
ATCAACG578400.033.1074033
CAACGCA598250.031.9074135
TATCAAC613600.031.2007352
AACGCAG621300.030.907376
ACGCAGA703450.026.9163977
CGCAGAG709350.026.4612798
GTACATG576700.025.006331
TACATGG568350.024.4609342
GCAGAGT766050.023.794419
ACATGGG601950.022.4302853
GAGTACT391700.020.21287312-13
AGAGTAC678500.020.0630210-11
CAGAGTA743400.019.41598710-11
GTACTTT408950.019.0318414-15
AGTACTT397750.018.81894512-13
CATGGGG341850.018.663374
CATGGGT145650.017.934084
Produced by FastQC (version 0.11.5)