FastQCFastQC Report
Thu 2 Jun 2016
DRR015429_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015429_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14748823
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT304950.20676226163945421No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218910.14842540316606959No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156160.10587963527665903No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA243600.044.6323931
GTATCAA463800.041.4245571
ATCAACG590250.032.1205333
TCAACGC590950.032.0489234
CAACGCA610100.031.0586835
TATCAAC628350.030.2672182
AACGCAG632300.030.0616766
ACGCAGA737600.025.5360537
CGCAGAG745450.025.19128
GTACATG534150.024.2923621
TACATGG530500.023.5018352
GCAGAGT803150.022.842529
ACATGGG557200.021.6720833
AGAGTAC706200.020.94192910-11
AGTACTT443950.019.74980512-13
GAGTACT435800.019.09570112-13
TATAACG11850.018.4651432
CATGGGT140400.018.3800644
CATGGGG312600.018.1730084
GTACTTT453800.018.04488414-15
Produced by FastQC (version 0.11.5)