FastQCFastQC Report
Thu 2 Jun 2016
DRR015428_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015428_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16064003
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT232110.14449075986850848No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187840.11693224907888775No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA244100.048.1387141
GTATCAA451650.040.7085271
ATCAACG523350.034.5488623
TCAACGC525000.034.4402774
CAACGCA549850.032.8639535
TATCAAC564050.032.1850242
AACGCAG579250.031.3307046
CGCAGAG645800.027.5657048
ACGCAGA650650.027.5499617
GTACATG556150.024.9582391
GCAGAGT721100.024.2062729
TACATGG559100.023.7440242
ACATGGG568850.022.7370283
GAGTACT373750.020.78281412-13
AGAGTAC647200.019.80770310-11
CAGAGTA702200.019.79164910-11
CATGGGG320250.018.5450824
GTACTTT420150.018.34060314-15
CATGGGT139600.018.1583844
AGTACTT400400.018.09866512-13
Produced by FastQC (version 0.11.5)