FastQCFastQC Report
Thu 2 Jun 2016
DRR015428_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015428_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16064003
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT235540.14662596863309849No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176040.10958663292082303No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA222600.047.1706541
GTATCAA425550.042.4322361
ATCAACG524750.033.7302973
TCAACGC529500.033.4393464
CAACGCA547850.032.3623625
TATCAAC564400.031.6030432
AACGCAG581850.030.5495036
CGCAGAG662250.026.4040838
ACGCAGA672650.026.2701477
GTACATG521700.024.780281
TACATGG527000.023.7405382
GCAGAGT733750.023.3342699
ACATGGG542500.022.3730583
AGAGTAC657050.020.64144110-11
GAGTACT396900.019.6367812-13
AGTACTT421900.019.22290212-13
CATGGGG308400.018.30474
CAGAGTA718200.018.2851210-11
CATGGGT134950.017.6719784
GTACTTT440350.017.52663814-15
Produced by FastQC (version 0.11.5)