FastQCFastQC Report
Thu 2 Jun 2016
DRR015427_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015427_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11233615
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193830.17254463500841002No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157290.1400172606947986No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA171700.051.7621271
GTATCAA321350.040.7277831
ATCAACG382200.033.7913673
TCAACGC384300.033.6067164
TATCAAC402000.032.2288972
CAACGCA401450.032.1579745
AACGCAG422450.030.6503436
CGCAGAG482200.026.4179338
ACGCAGA485350.026.331317
GTACATG399250.023.6740061
GCAGAGT526500.023.4505169
TACATGG396800.022.891432
ACATGGG417700.021.1577173
GAGTACT270450.020.71479412-13
CAGAGTA511900.019.62696810-11
AGAGTAC477350.019.61800810-11
GTGGTAT82150.018.5020161
GTACTTT306200.017.9390614-15
AGTACTT293750.017.56177512-13
CATGGGG236200.017.1472364
Produced by FastQC (version 0.11.5)