FastQCFastQC Report
Thu 2 Jun 2016
DRR015427_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015427_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11233615
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195910.1743962206288893No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125240.11148681880231787No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115020.10238912407092463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA130650.047.7565881
GTATCAA272900.045.860331
ATCAACG371700.033.082763
TCAACGC374550.032.786454
TATCAAC391150.031.6008452
CAACGCA390550.031.4854975
AACGCAG414000.029.8229986
ACGCAGA494450.024.7129047
CGCAGAG493600.024.5191278
GTACATG336050.022.4485451
TACATGG339050.021.8713782
GCAGAGT537000.021.7226569
AGAGTAC489750.020.90950810-11
ACATGGG366850.019.7542673
AGTACTT319200.019.6695112-13
CAGAGTA524200.018.6932879
GAGTACT292750.018.2984512-13
TATAACG11000.017.4023912
ATAACGC12650.017.2943023
CATGGGG214250.016.2334294
Produced by FastQC (version 0.11.5)