FastQCFastQC Report
Thu 2 Jun 2016
DRR015426_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015426_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12578346
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230490.18324348845229732No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181300.14413659792789926No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA174100.051.5706181
GTATCAA332150.042.9102941
ATCAACG394050.035.7889863
TCAACGC394050.035.7889864
CAACGCA411700.034.1633955
TATCAAC422100.033.4969372
AACGCAG433700.032.6653636
ACGCAGA489700.028.595447
CGCAGAG494550.028.185778
GCAGAGT547000.024.8909479
GTACATG380100.023.4057671
TACATGG376650.022.5330522
ACATGGG383500.021.2643363
CAGAGTA533650.020.42454110-11
GAGTACT327250.020.26720412-13
AGAGTAC504800.019.93369510-11
TATAACG12500.019.295012
GTACTTT351450.018.66407614-15
GTGGTAT80550.018.5302931
AGTACTT341900.018.1414512-13
Produced by FastQC (version 0.11.5)