FastQCFastQC Report
Thu 2 Jun 2016
DRR015426_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015426_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12578346
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225110.17896629652261115No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170120.13524830689186002No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA160400.049.7724651
GTATCAA314000.043.9056361
ATCAACG389500.034.759383
TCAACGC392800.034.481444
CAACGCA406400.033.2685365
TATCAAC420100.032.487942
AACGCAG427300.031.8010646
ACGCAGA490050.027.5993187
CGCAGAG490950.027.3849228
GCAGAGT543650.024.2359819
GTACATG353400.022.5389211
TACATGG348650.021.8348312
AGAGTAC508400.020.84936110-11
TATAACG12500.020.4221322
ACATGGG356950.020.3693123
GTGGTAT77700.019.786291
GAGTACT345450.019.15602712-13
CAGAGTA534100.018.98547410-11
AGTACTT362500.018.8451212-13
GTACTTT369350.017.6801714-15
Produced by FastQC (version 0.11.5)