FastQCFastQC Report
Thu 2 Jun 2016
DRR015425_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015425_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9755045
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169250.17349996847784915No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127700.13090662318830923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA135600.048.361511
TATAACG11250.044.902272
GTATCAA260300.042.0121771
TACCTGG100050.035.1154332
TCAACGC308950.035.0432244
ATCAACG309100.034.95263
CAACGCA323800.033.572845
TATCAAC330000.032.8768462
ATAACGC15600.032.0897183
AACGCAG345250.031.7967976
GTACCTG114950.031.5929011
TAACGCA18800.029.0483344
ACCTGGG124750.027.7979153
ACGCAGA390700.027.7951347
CGCAGAG394400.027.4184658
GTACATG327850.025.8741631
TACATGG330450.024.6791422
GCAGAGT431950.024.2543169
ACATGGG334050.023.3233053
GTATAAC30550.022.9406171
Produced by FastQC (version 0.11.5)