FastQCFastQC Report
Thu 2 Jun 2016
DRR015425_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015425_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9755045
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175630.18004017408428152No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120850.1238846156014657No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA124000.049.4579661
GTATCAA247600.042.5163151
TATAACG10500.037.7761732
TACCTGG97250.035.864092
TCAACGC308150.033.5748374
ATCAACG310600.033.3792843
CAACGCA321850.032.3095865
GTACCTG110600.032.098381
TATCAAC330700.031.5434972
AACGCAG345950.030.4402836
ACCTGGG118950.027.596633
ATAACGC15450.026.5584073
ACGCAGA403100.025.9218927
CGCAGAG406350.025.6050668
GTACATG299950.025.1315821
TACATGG308750.023.4493892
GCAGAGT444400.022.8798089
ACATGGG314500.022.049393
GTATAAC28200.020.711881
AGAGTAC410050.020.64505410-11
Produced by FastQC (version 0.11.5)