FastQCFastQC Report
Thu 2 Jun 2016
DRR015424_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015424_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12021403
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223390.1858268955794927No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176880.14713756788621096No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA176000.051.5851631
GTATCAA345200.042.3447271
ATCAACG412450.034.9225543
TCAACGC411900.034.8918464
CAACGCA429550.033.4980245
TATCAAC447350.032.289632
AACGCAG450950.032.0415086
ACGCAGA509600.028.1217277
CGCAGAG508850.028.0283668
GCAGAGT559500.024.7903189
GTACATG398500.024.1418861
TACATGG393400.023.2869722
ACATGGG402850.021.9838163
GAGTACT338100.020.20896312-13
AGAGTAC522200.020.2016910-11
GTGGTAT81950.019.9916251
CAGAGTA556900.019.8986610-11
GTACTTT352750.019.03413814-15
TGGTATC80400.018.8491522
AGTACTT353950.017.96094512-13
Produced by FastQC (version 0.11.5)