FastQCFastQC Report
Thu 2 Jun 2016
DRR015424_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015424_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12021403
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229600.19099268196898483No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164250.13663130667859652No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA165000.049.017591
GTATCAA331050.043.529451
ATCAACG422350.033.6620373
TCAACGC425950.033.2409134
CAACGCA439900.032.0899475
TATCAAC452650.031.539642
AACGCAG459900.030.8230636
ACGCAGA531150.026.4490227
CGCAGAG529350.026.3275418
GCAGAGT575350.023.6838049
GTACATG369700.023.5550291
TACATGG364900.022.772482
ACATGGG378200.021.3087643
AGAGTAC538950.021.09472710-11
AGTACTT375600.019.24818612-13
GAGTACT359350.019.02306212-13
CAGAGTA581150.018.25900710-11
TATAACG11600.018.0757942
GTACTTT374500.017.98333714-15
CATGGGA159350.016.1345624
Produced by FastQC (version 0.11.5)