FastQCFastQC Report
Thu 2 Jun 2016
DRR015423_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015423_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8730385
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT364170.41712937058331334No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT322940.3699035036828273No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148630.17024449666309102No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA189250.060.2897531
GTATCAA354600.040.2207951
TCAACGC430700.032.7230224
ATCAACG430400.032.714123
CAACGCA449500.031.4353715
TATCAAC454000.031.183912
GTGGTAT61800.030.9180891
AACGCAG460700.030.808346
ACGCAGA506700.027.4997067
CGCAGAG503600.027.3607568
TGGTATC67750.027.3295172
GCAGAGT540400.024.790179
GTACATG308050.023.2305411
TACATGG302300.022.362872
GAGTACT350500.021.94680812-13
CAGAGTA525100.021.91772810-11
GTACTTT363300.020.8479514-15
ACATGGG315850.020.6689243
AGAGTAC505300.020.23333210-11
AGTACTT354600.019.55068812-13
Produced by FastQC (version 0.11.5)