FastQCFastQC Report
Thu 2 Jun 2016
DRR015423_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015423_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8730385
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT279340.3199629798685854No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT243930.27940348564238576No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139130.1593629605109053No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA153150.069.814581
GTATCAA287100.047.0009881
ATCAACG380400.035.0588873
TATCAAC383000.034.992562
TCAACGC403850.032.430654
GTGGTAT57450.031.1140171
CAACGCA433850.030.229695
AACGCAG448400.029.2990536
TGGTATC61800.028.282662
ACGCAGA494850.026.156037
CGCAGAG492400.025.9875958
GCAGAGT523550.023.619539
GTACATG257500.023.1462611
TACATGG259750.021.4316792
CAGAGTA516400.021.07700510-11
GAGTACT362650.021.05454812-13
AGAGTAC492050.020.77748110-11
GTACTTT372150.020.07800114-15
AGTACTT367250.019.67582312-13
ACATGGG271400.019.603223
Produced by FastQC (version 0.11.5)