FastQCFastQC Report
Thu 2 Jun 2016
DRR015422_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015422_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11183300
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT374440.3348206701063193No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT302800.270760866649379No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158950.14213157118203035No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA204800.056.02191
GTATCAA389950.041.9520761
ATCAACG467300.034.4919743
TCAACGC467800.034.464994
CAACGCA485600.033.2110185
TATCAAC494150.032.765132
AACGCAG503250.032.0912976
ACGCAGA562600.028.1883957
CGCAGAG562450.027.8635438
GCAGAGT600150.025.172969
GTACATG334550.024.6807481
GTGGTAT79000.024.4163911
TACATGG333600.023.3191552
TGGTATC83750.021.6192672
ACATGGG343750.021.598333
GAGTACT395200.021.35219412-13
CAGAGTA585650.020.933610-11
AGAGTAC549250.020.44496310-11
GTACTTT423950.019.71066714-15
TATAACG10500.019.0636522
Produced by FastQC (version 0.11.5)