FastQCFastQC Report
Thu 2 Jun 2016
DRR015422_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015422_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11183300
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT316260.282796670034784No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239420.21408707626550302No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156700.1401196426814983No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA173200.060.369231
GTATCAA335350.048.9191171
ATCAACG433150.037.424763
TATCAAC437000.037.269962
TCAACGC462000.034.9313744
CAACGCA493600.032.8182225
AACGCAG517550.031.4130526
ACGCAGA576250.027.7352757
CGCAGAG576950.027.525428
GTACATG297050.025.6178761
GCAGAGT616450.025.0261829
GTGGTAT79450.024.8218941
TACATGG299150.023.799932
ACATGGG306500.022.1425553
TGGTATC85150.021.801252
AGAGTAC570700.021.1247610-11
TATAACG9950.020.8519952
GAGTACT425050.020.64976912-13
CAGAGTA611800.019.93123210-11
AGTACTT445200.019.84221312-13
Produced by FastQC (version 0.11.5)