FastQCFastQC Report
Thu 2 Jun 2016
DRR015421_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015421_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12504270
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT492650.3939854145823787No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350290.28013630543806234No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210680.1684864450303776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA211950.059.5640831
GTATCAA445300.044.768711
ATCAACG534300.036.8773463
TCAACGC537500.036.666414
CAACGCA555700.035.465535
TATCAAC571950.034.4975282
AACGCAG580400.034.0182576
ACGCAGA652200.029.6803137
CGCAGAG654250.029.3775338
GCAGAGT697800.026.728739
GTGGTAT73150.026.3075831
GTACATG438150.025.2623771
TACATGG431000.024.1826822
ACATGGG449600.022.2917843
TGGTATC82100.021.5003362
CAGAGTA679050.021.40067310-11
TATAACG10850.021.3844032
GAGTACT456150.021.00752412-13
AGAGTAC644900.020.81275210-11
GTACTTT468800.020.15885414-15
Produced by FastQC (version 0.11.5)