FastQCFastQC Report
Thu 2 Jun 2016
DRR015421_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015421_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12504270
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT426600.34116345856255503No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT287620.23001742604726225No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209150.16726286300599713No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA180250.064.502131
GTATCAA374950.052.589841
ATCAACG498350.039.2248273
TATCAAC508300.038.60762
TCAACGC537050.036.5141334
CAACGCA576100.034.061835
AACGCAG602050.032.661316
ACGCAGA671300.028.7872247
CGCAGAG674800.028.2860478
GTGGTAT67700.027.9626181
GTACATG370350.025.9714951
GCAGAGT714100.025.8666869
TACATGG372200.024.2249972
ACATGGG384400.022.4888863
TGGTATC82300.021.7918192
AGAGTAC677950.021.52248610-11
AGTACTT502550.020.37219812-13
GAGTACT497050.020.12421812-13
CAGAGTA712300.020.11612110-11
GTACTTT504650.019.56870714-15
Produced by FastQC (version 0.11.5)