FastQCFastQC Report
Thu 2 Jun 2016
DRR015420_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015420_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10007666
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT361180.36090333150606746No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT304130.3038970325348588No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172500.17236786279638028No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA191250.053.095121
GTATCAA347600.039.7359731
TCAACGC426700.032.1346664
ATCAACG428850.031.9523453
TATCAAC447450.030.7359642
CAACGCA446750.030.723025
AACGCAG461850.029.8657056
ACGCAGA506250.026.6712517
CGCAGAG509750.026.1569988
GTACATG311600.025.7696881
TACATGG313300.024.1191752
GCAGAGT538350.023.8882269
ACATGGG327500.022.4205063
GAGTACT339000.021.38949812-13
GTGGTAT83800.021.0644231
AGAGTAC474300.020.54883810-11
CAGAGTA523100.020.49284610-11
TATAACG7850.020.2839052
GTACTTT362900.019.67973314-15
CATGGGG205900.019.3995364
Produced by FastQC (version 0.11.5)