FastQCFastQC Report
Thu 2 Jun 2016
DRR015420_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015420_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10007666
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT294200.2939746390417106No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233190.23301137348108938No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167540.16741166221974232No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA156500.060.207851
GTATCAA295650.047.611831
ATCAACG391450.035.6035653
TATCAAC393600.035.4784362
TCAACGC413850.032.646524
CAACGCA448000.030.2385965
AACGCAG464000.029.2446426
GTACATG267700.026.9545941
ACGCAGA511150.026.034277
CGCAGAG516200.025.4594468
TACATGG277000.024.3589022
GCAGAGT545550.023.324959
ACATGGG286100.022.8017963
AGAGTAC497850.021.22859810-11
GAGTACT362500.020.74112512-13
GTGGTAT71500.020.4742071
AGTACTT375350.020.320412-13
CAGAGTA542100.019.41229410-11
GTACTTT389250.019.22126214-15
CATGGGG192250.018.4120664
Produced by FastQC (version 0.11.5)