FastQCFastQC Report
Thu 2 Jun 2016
DRR015419_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015419_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9682345
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228630.23613081335151762No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185720.19181303702770353No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA162850.055.8997761
GTATCAA313750.042.4704061
ATCAACG372450.035.1289333
TCAACGC372450.035.1045044
CAACGCA388400.033.7214745
TATCAAC392650.033.5186772
AACGCAG405800.032.4309966
ACGCAGA445800.028.8782047
CGCAGAG440650.028.7815288
GTGGTAT67550.025.7943271
GCAGAGT466300.025.6175429
GTACATG308250.023.4958291
TGGTATC68550.023.2939822
TACATGG309600.021.835442
GAGTACT271750.021.33441412-13
CAGAGTA452900.021.01233110-11
ACATGGG322300.020.2410433
GTACTTT285600.020.02878614-15
AGAGTAC423300.019.94548410-11
AGTACTT285850.018.38036712-13
Produced by FastQC (version 0.11.5)