FastQCFastQC Report
Thu 2 Jun 2016
DRR015419_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015419_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9682345
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174300.18001837364811935No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130920.1352151777281227No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA129550.060.8603861
GTATCAA252450.049.5437771
ATCAACG323300.038.393343
TATCAAC328550.037.812972
TCAACGC344200.035.5358774
CAACGCA371850.032.9931455
AACGCAG391100.031.4616266
ACGCAGA433050.027.8812397
CGCAGAG432100.027.4648848
GCAGAGT456350.024.87369
GTGGTAT57000.023.881891
GTACATG262050.022.2124821
GAGTACT282350.020.5098112-13
AGAGTAC425400.020.40079310-11
TACATGG270200.020.3410022
TGGTATC63300.020.2022842
CAGAGTA454800.019.75870910-11
GTACTTT293300.019.24831814-15
AGTACTT295850.018.87020912-13
ACATGGG283100.018.7990253
Produced by FastQC (version 0.11.5)