FastQCFastQC Report
Thu 2 Jun 2016
DRR015418_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015418_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16068702
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT380280.2366588166237696No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT274230.17066095319958016No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA237050.054.2167471
GTATCAA487950.045.8343961
ATCAACG584100.037.6809163
TCAACGC584150.037.662354
CAACGCA621000.035.383525
TATCAAC628750.035.2655262
AACGCAG645100.034.2166826
ACGCAGA707900.030.4743047
CGCAGAG709050.030.012578
GCAGAGT764000.026.7283429
GTACATG568550.025.5976751
TACATGG564550.024.1102662
ACATGGG576600.022.7621863
GTGGTAT97700.022.4443721
CAGAGTA737400.020.68767410-11
GAGTACT434750.020.55531512-13
AGAGTAC696200.020.46137610-11
GTACTTT443800.019.75671414-15
TGGTATC105250.019.580282
AGTACTT444050.019.01331312-13
Produced by FastQC (version 0.11.5)