FastQCFastQC Report
Thu 2 Jun 2016
DRR015418_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015418_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16068702
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT289430.1801203357931462No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197280.12277282881965203No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA187700.059.0235821
GTATCAA394000.053.1481441
ATCAACG504850.041.14033
TATCAAC533850.039.027522
TCAACGC536350.038.2494434
CAACGCA578550.035.4427455
AACGCAG602000.034.0844656
ACGCAGA673800.029.8082087
CGCAGAG677600.029.2369088
GTACATG505500.026.8607231
GCAGAGT730950.026.1171369
TACATGG518100.024.8913482
ACATGGG532850.023.4686093
GTGGTAT88250.022.2877641
AGAGTAC678400.020.4713710-11
CAGAGTA720650.019.5726410-11
GAGTACT418650.019.52420412-13
AGTACTT435550.018.75624312-13
CATGGGG322650.018.6141434
GTACTTT434200.018.38570214-15
Produced by FastQC (version 0.11.5)